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2010 (Vol. 2, Issue: 2)
Article Information:

Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment

G. Rajaram, P. Manivasagan, B. Thilagavathi, A. Saravanakumar
Corresponding Author:  Manivasagan 

Key words:  Antagonistic activity, bacteriocin, indicator organisms, lactic acid bacteria, Lactobacillus lactis, ,
Vol. 2 , (2): Page No: 138-144
Submitted Accepted Published
2009 December, 30 2010 January, 16 2010 March, 10

Bacteriocin producing Lactobacillus lactis strain isolated from marine environment, showed broad range of antibacterial activity against some major food borne pathogens. Maximum bacteriocin production was observed at 30C , pH 6.0 and 1.5% sodium chloride solution. In addition of enzymes, "α-amylase, DNase, RNase and lipase were slightly positive effect bacteriocin production. Proteinase K and pepsin were strongly inhibited bacteriocin production. Among detergents, Sodium dodecyl sulphate (SDS), Tween 80 and Tritone X-100 stimulated bacteriocin production and strongly inhibited by EDTA and urea. The bacteriocin has purified by ammonium sulphate precipitate and ion exchange (DEAE cellulose) chromatography. Biochemically it was pure protein moiety and the molecular weight was 94 kDA. The study revealed the possibility of using bacteriocin as a food preservative and the L. lactis strain as probiotic.
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  Cite this Reference:
G. Rajaram, P. Manivasagan, B. Thilagavathi, A. Saravanakumar, 2010. Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment.  Advance Journal of Food Science and Technology, 2(2): Page No: 138-144.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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