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2013 (Vol. 5, Issue: 06)
Article Information:

Preparation and Enzyme-Linked Immunosorbent Assay of Monoclonal Antibody Against Ampicillin

Yan Lu, Xuping Zhou, Hongyu Zhao, Jian Sun and Guojuan Wu
Corresponding Author:  Guojuan Wu 

Key words:  Ampicillin, ELISA, monoclonal antibody, , , ,
Vol. 5 , (06): 783-786
Submitted Accepted Published
February 25, 2013 March 27, 2013 June 05, 2013
Abstract:

Ampicillin (AMP) was coupled with mcKLH by the method of EDC intermediate and injected into Balb/c mice as immunogen. The hybridomas were obtained by fusing mouse myeloma cells SP2/0 with splenocytes from the mice immunized with AMP-KLH. The chromosomal numbers were 97-104, the results indicated that the subclass of the McAb was IgG2a, the titer of ascitic fluid was 2106, the affinity constant was 310-10 mol/L, the molecular weight was 154KD. The cross-reactivities of McAb to other antibiotics were below 0.01%. The linear detecting range of calibration curves to detect AMP was 0.5 ng/mL-100 ng/mL. The formular was Y = 0.0569X+0.1308, R2 = 0.9948. The limit of detection was 0.3 ng/mL.
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  Cite this Reference:
Yan Lu, Xuping Zhou, Hongyu Zhao, Jian Sun and Guojuan Wu, 2013. Preparation and Enzyme-Linked Immunosorbent Assay of Monoclonal Antibody Against Ampicillin.  Advance Journal of Food Science and Technology, 5(06): 783-786.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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