Abstract
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Article Information:
A Validated RP-LC Method for the Determination of Erythromycin an Oxime and Related Substances
Zhiling Cao, Bing Liu, Weiwei Liu, Yinxing SU, Wenming Bo and Guowei Yao
Corresponding Author: Zhiling Cao
Submitted: October 09, 2012
Accepted: November 23, 2012
Published: January 15, 2013 |
Abstract:
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A liquid chromatographic method was developed and validated for the determination of erythromycin an oxime and related substances. The chromatographic analysis was performed on Inertsil C18 ODS column, using the phosphate buffer solution (0.02 M, pH 6.5)-acetonitrile (40:60, V/V) as the mobile phase. Wavelength of the UV detector was 215 nm. All four related substances, including Z isomer of erythromycin A oxime, erythromycin A, erythromycin A-6, 9-hemiketal and erythromycin A-6, 9-9, 12-spiroketal were separated and determined under isocratic elution. The effect of pH and the concentration of mobile phase on separation were studied. The developed method was successfully applied to the analysis of erythromycin an oxime in commercial formulation.
Key words: Column liquid chromatography, erythromycin an oxime, macrolide antibiotics, related substance, , ,
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Cite this Reference:
Zhiling Cao, Bing Liu, Weiwei Liu, Yinxing SU, Wenming Bo and Guowei Yao , . A Validated RP-LC Method for the Determination of Erythromycin an Oxime and Related Substances. Advance Journal of Food Science and Technology, (01): 68-71.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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