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    Abstract
2012 (Vol. 4, Issue: 6)
Article Information:

Studies on the Interactions between Pto and Pti1s Proteins from Maize by a Yeast Two-Hybrid System

Yong Zhu, Xiao-yan Sun, Beng Wang, Zhong-jian Song, Hua-wen Zou
Corresponding Author:  Hua-wen Zou 

Key words:  Maize, Yeast two-hybrid system, ZmPto, ZmPti1, , , ,
Vol. 4 , (6): 389-392
Submitted Accepted Published
September 11, 2012 October 19, 2012 December 20, 2012
Abstract:

To check whether the protein ZmPto can interact with the protein ZmPti1s, in this study, the gene sequences of ZmPto and ZmPti1a, ZmPti1b were inserted into yeast expression vectors pEG202 and pJG4-5, respectively. Yeast two-hybrid was then conducted to verify their interactions. Results showed that yeast strains neither carrying empty plasmid nor carrying only one single fusion sequence of ZmPti1 or ZmPto can activate reporter genes. Only the yeast strains carrying both fusion sequence of ZmPti1 and ZmPto can activate reporter genes indicating that ZmPto can interact directly with ZmPti1 in the yeast two-hybrid system.
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  Cite this Reference:
Yong Zhu, Xiao-yan Sun, Beng Wang, Zhong-jian Song, Hua-wen Zou, 2012. Studies on the Interactions between Pto and Pti1s Proteins from Maize by a Yeast Two-Hybrid System.  Advance Journal of Food Science and Technology, 4(6): 389-392.
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