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Abstract
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Article Information:
Antagonistic Potential of Lactobacillus Spp against Enteropathogenic Bacteria; Purification and Characterization of their Bacteriocins
Asha and D. Gayathri
Corresponding Author: D. Gayathri
Submitted: July 20, 2012
Accepted: August 28, 2012
Published: October 20, 2012 |
Abstract:
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In the present study, Lactobacillus (160) isolates were isolated from curd sample. The isolates were
aimed to analyze the antibacterial potential against Escherichia coli, Vibrio cholerae sub sp., ogawa, V. cholerae sub
sp., inaba, Klebsiella sp., Proteus sp. and Shigella dysenteriae. All the isolates were inhibiting the tested
Enteropathogenic bacteria except S. dysenteriae. Lactobacillus isolates produced highest inhibition zone (30 to 37
mm) against V. cholerae sub sp., inaba and Klebsiella sp., of the 160 isolates only ten Lactobacillus isolates (L1-
L10) were used for the production of bacteriocins, purified by ammonium sulphate precipitation and ion exchange
(DEAE cellulose) chromatography. Maximum bacteriocin activity has been observed with Lf3 against V. cholerae
ssp Inaba at 30°C, pH 6.0, 1.5 to 2.0% Na Cl/18 h in addition to L8, L9 and L10 (MW 100 to 106 KDa) and Lf3
was found to be the most prominent potential isolate.
Key words: Bacteriocins, enteropathogens, inhibition zone, Lactobacillus fermentum, Lactobacillus plantarum, SDS-PAGE,
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Abstract
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Cite this Reference:
Asha and D. Gayathri, . Antagonistic Potential of Lactobacillus Spp against Enteropathogenic Bacteria; Purification and Characterization of their Bacteriocins. Advance Journal of Food Science and Technology, (5): 265-269.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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