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Abstract
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Article Information:
Supplementation of Indigenous Lactobacillus Bacteria in Live Prey and as Water Additive to Larviculture of Portunus pelagicus (Linnaeus, 1758)
A.D. Talpur, A.J. Memon, M.I. Khan, M. Ikhwanuddin, M.M. Danish Daniel and A.B. Abol-Munafi
Corresponding Author: A.D. Talpur
Submitted: 2011 August, 01
Accepted: 2011 September, 08
Published: 2011 October, 25 |
Abstract:
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Experimental trials were conducted to demarcate the effects of indigenous Lactobacillus probiotics
as bioencapsulated in live prey (rotifers Brachionus plicatilis and Artemia franciscana) and water additives
together on the survival of blue swimming crab, Portunus pelagicus larvae. Three LAB probiotics L. plantarum,
L. salivarius and L. rhamnosus at final concentration 1x107 cfu/mL were bioencapsulated in live prey added
daily and same allowance was added to culture water on day 1, 3, 5, 7, 9, 11, 13 as a single isolates to
treatments A, B, C and multi isolates to D with no probiotic added to control tanks. Bacteria were successfully
accumulated in both rotifers and Artemia within two hours of incubation. Total viable count of bacteria in
Artemia observed lower at sampling days in inoculated tanks compared to those at time of incubation, contrary
it increased in controls and no Vibrio was determined in Artemia in LAB mixture isolate inoculated samples
on the day 13. Highest LAB bacteria 4.10×103 was determined in Artemia on day 11 in those inoculated with
mixture of LAB isolates. At the end of the trials, larvae treated with a mixture of LAB probiotics did produce
significantly highest survival 13.83±0.76% over other LAB treatments. As a single isolates L. plantarum did
produce survival 13.50±1.32% compared with those treated with L. salivarius and L. rhamnosus and those
without probiotics (control). There was no statistical significance (p>0.05) in the survival of larvae in any
treatment. Results indicate that LAB probiotics could be used to enhance survival of P. pelagicus larvae.
Key words: Accumulated, Artemia franciscana, Brachionus plicatilis, Lactobacillus, significance, ,
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Cite this Reference:
A.D. Talpur, A.J. Memon, M.I. Khan, M. Ikhwanuddin, M.M. Danish Daniel and A.B. Abol-Munafi, . Supplementation of Indigenous Lactobacillus Bacteria in Live Prey and as Water Additive to Larviculture of Portunus pelagicus (Linnaeus, 1758). Advance Journal of Food Science and Technology, (5): 390-398.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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