Identification of RAPD Primers Specific for Wheat Homoeologous Group 1 and 3 Chromosomes
Ikram Muhammad, Habib Ahmad, Sajid Ghafoor, Sahib Gul Afridi, Khaist Begum and Imtiaz Ahmad Khan
Corresponding Author: Imtiaz Ahmed Khan
Key words: Homoeolgous chromosomes, mapping, Nullisomic-tetrasomic lines, RAPD, Triticun aestivum, ,
Vol. 2 , (3): Page No: 117-119
|2009 December, 30
||2010 January, 25
||2010 July, 05
Mapping of homoeologous chromosomes of common wheat (Triticum aestivum L.) has always been
a top priority for wheat breeders. During present work, easier, cheaper and quicker RAPD (Randomly
Amplified Polymorphic DNA) primers were mapped on homoeologous group 1 and 3 chromosomes of common
wheat. Wheat genetic stocks viz; Nullisomic-tetrasomic lines were used for mapping of RAPD primers. A total
of 43 RAPD primers were tested, out of which, five primers viz; GLA-10, GLA-11, GLA-14, GLA-20 and
GLB-15 were found useful and these primers amplified DNA fragments specific for chromosomes 1D, 3D, 1B,
3B and 1D, respectively. These five RAPD primers can reliably be used as markers for the wheat
homoeologous chromosomes 1B, 3B, 1D and 3D.
Cite this Reference:
Ikram Muhammad, Habib Ahmad, Sajid Ghafoor, Sahib Gul Afridi, Khaist Begum and Imtiaz Ahmad Khan, 2010. Identification of RAPD Primers Specific for Wheat Homoeologous Group 1 and 3 Chromosomes.
Asian Journal of Agricultural Sciences, 2(3): Page No: 117-119.