In order to establish an efficient in vitro propagation protocol for Iranian edible wild A sparagus, this experiment was carried out. For this purpose, explants were excised from spear lateral buds and cultured on MS medium containing 6% sucrose and different NAA and BAP concentrations for both callus induction and shoot development. The treatment inducing the form ation of the highest number of shoots was achieved on a medium containing 0.015 mg/l NAA and 0.5 mg/l BAP. For root formation, media w ere supplemented with two IBA concentrations (1.25 and 1.5 mg/l), that 1.5 mg/l giving a higher rooting percentage (43%). These preliminary results showed that the treatment containing 0.015 mg/l NAA, 0.5 mg/l BAP and 1.5 mg/l IBA gave the highest number of plantlets. Also, The data present a relatively efficient method for in vitro culture of this valuable vegetable in Persia.