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     Asian Journal of Agricultural Sciences

    Abstract
2012(Vol.4, Issue:6)
Article Information:

″Teucrium polium″ Extracts Jordanian Ja’adeh

Nawal Hassan Al Bahtiti
Corresponding Author:  Nawal Hassan Al Bahtiti 
Submitted: July 26, 2012
Accepted: August 28, 2012
Published: November 25, 2012
Abstract:
This research is intended to record the possible variations in the constituents, chemical composition and antimicrobial activity extracts of cyclohexane, dichloromethane and ethanol extracts of Teucrium polium (Jordanian Jeadaha). That collected on locality Almojub Valley (southern deserts of Jordan). Cyclohexane extracts of T. polium possessed high activity against Bacillus subtilis (MIC = 65 &mug/mL Dichloromethane extract was more effective against Bacillus subtilis (MIC = 32.5&mug/mL). The ethanol extract of T. polium no activity against Bacillus subtilis Herb extracts of T. polium have shown weak antibacterial activity on Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Staphylococcus aurous. The antioxidant activities of the extracts were evaluated using 2 different tests: the thiocyanate method and scavenging of the 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH) radical. The cyclohexane, dichloromethane and ethanol extracts had antioxidant activities. The flavonoid contents of all of the extracts were also determined. The amounts of total flavonoids were higher in dichloromethane and ethanol extracts. The extracts, were subjected to chromatographic methods for the isolation of the active compounds. The structures of isolated compounds were characterized by UV, IR, and 13C NMR, spectroscopic methods as cirsilineol (1), luteolin-7-O-rutinoside (2), luteolin-7-O-glucoside (3), hesperetin-7-O-rutinoside (4), apigenin (5) diosmetin (6), Luteolin-7-sambubioside (7), Diosmetin 7-O-rutinoside, (8) and Nicotiflorin (9) (Fig. 1). These isolated compounds were also tested for their antioxidant and DPPH radical scavenging activities. Flavonoids showed potent antioxidant and DPPH radical scavenging activities. The highest antioxidant activity was shown by luteolin-7-O-glucoside (Djilas et al., 2006). This study found that the total phenolic content of the extracts ranged from 50.0 mg/g to 170.0 mg GA/g. The present results therefore offer a scientific basis for traditional use of Teucrium polium for the treatment of bacterial and fungal infections.

Key words:  Antibacterial activity, extracts, flavonoids, flavonoids, phenols, spectroscopic methods, Teucrium polium
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Cite this Reference:
Nawal Hassan Al Bahtiti, . ″Teucrium polium″ Extracts Jordanian Ja’adeh. Asian Journal of Agricultural Sciences, (6): 379-382.
ISSN (Online):  2041-3890
ISSN (Print):   2041-3882
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