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Abstract
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Article Information:
Molecular Prediction of Pea Footrot Disease
Ebimieowei Etebu and A. Mark Osborn
Corresponding Author: Ebimieowei Etebu
Submitted: 2011 April, 06
Accepted: 2011 May, 13
Published: 2011 November, 15 |
Abstract:
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PCR based assays were developed in this study to quantitatively predict pea footrot infections in
agricultural soils prior to cultivation. Pea footrot disease due to Nectria haematococca (anamorph Fusarium
solani f.sp. pisi) is linked to the presence of six pea pathogenicity (PEP) genes (PDA1, PEP1, PEP2, PEP3,
PEP4 and PEP5). Whilst molecular assays have been used recently to selectively detect these genes in soil-
DNA, quantitative molecular assay has been extended to only the PEP3 gene whose role in pea pathogenicity
is yet unknown. In this research, PCR-based quantification assays were developed to quantify the two pea
pathogenicity genes (PDA and PEP5) with identified roles in pea pathogenicity from soil-DNA obtained from
fields with pea footrot histories. Results showed that the quantitative molecular assays developed herein were
both efficient. Amplification efficiency of the Q-PCR assay for the PDA and PEP5 gene were 97 and 89%,
respectively. PDA and PEP5 gene copy numbers were shown to vary significantly (p = 0.01) between fields.
However, the PDA gene copy numbers were relatively higher than those of the PEP5 gene in agricultural fields.
The genes, especially PEP5 gene, were comparable to and positively correlated to the number of spores of
pathogenic N. haematococca, and footrot disease. The PDA gene alone in soil could not cause footrot disease
in peas after 8 weeks of planting; assays directed at it alone may therefore be insufficient to predict pea footrot
disease. However, the molecular assay targeting the PDA alongside the PEP5 gene offers the opportunity for
quantitative prediction of pea footrot infections in agricultural soils prior to cultivation.
Key words: Footrot disease, Nectria haematococca, PDA, peas, pea pathogenicity genes, PEP5, soil-DNA
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Cite this Reference:
Ebimieowei Etebu and A. Mark Osborn, . Molecular Prediction of Pea Footrot Disease. Asian Journal of Agricultural Sciences, (6): 417-426.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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